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Record Details

Record 28 of 1009
Changes in diaphragm muscle collagen gene expression after acute unilateral denervation
Author and Affiliation:
Gosselin, L. E.(Mayo Clinic and Foundation, Department of Anesthesiology, Rochester, Minnesota 55905, United States)
Sieck, G. C.
Aleff, R. A.
Martinez, D. A.
Vailas, A. C.
Abstract: The purpose of the present study was to examine the effects of acute (3 days) unilateral diaphragm denervation (DNV) on 1) levels of alpha 1(I) and alpha 1(III) procollagen mRNA; 2) collagen concentration [hydroxyproline (HYP)]; 3) amount of the nonreducible collagen cross-link hydroxylysylpyridinoline (HP); and 4) the passive force-length relationship of the muscle. The levels of alpha 1(I) and alpha 1(III) procollagen mRNA, HYP concentration, and amount of HP were measured in muscle segments from the midcostal region of DNV and intact (INT) hemidiaphragms of adult male Fischer 344 rats (250-300 g). The in vitro passive force-length relationship of DNV and INT hemidiaphragm was determined by lengthening and shortening the diaphragm muscle segments from 85 to 115% of optimal length at a constant velocity (0.6 optimal length/s). Three days after DNV, the level of alpha 1(I) procollagen mRNA was increased over 15-fold in the DNV hemidiaphragm compared with INT (P < 0.05), whereas the level of alpha 1(III) procollagen mRNA was increased by approximately sixfold in the DNV hemidiaphragm compared with INT (P < 0.05). Collagen (HYP) concentration did not differ between groups, averaging 8.7 and 8.9 micrograms/mg dry wt for the DNV and INT hemidiaphragms, respectively. In addition, there was no difference in the amount of the mature nonreducible collagen cross-link HP between the DNV and INT hemidiaphragms (0.66 vs. 0.76 mole HP/mole collagen, respectively). The amount of passive force developed during lengthening did not differ between DNV and INT hemidiaphragms. These data indicate that acute DNV of the hemidiaphragm is associated with an increase in the mRNA level of the two principal fibrillar collagen phenotypes in skeletal muscle. However, despite extensive muscle remodeling, the passive force-length relationship of the DNV hemidiaphragm is unaffected compared with the INT muscle.
Publication Date: Oct 01, 1995
Document ID:
20050000133
(Acquired Jan 06, 2005)
Subject Category: LIFE SCIENCES (GENERAL)
Document Type: Journal Article
Publication Information: Journal of applied physiology (Bethesda, Md. : 1985) (ISSN 8750-7587); Volume 79; 4; 1249-54
Publisher Information: United States
Contract/Grant/Task Num: HL-34817; HL-37680
Description: In English
Distribution Limits: Unclassified; Publicly available; Unlimited
Rights: Copyright
NASA Terms: BIOSYNTHESIS; COLLAGENS; DIAPHRAGM (ANATOMY); GENE EXPRESSION; MUSCLES; AMINO ACIDS; AUTORADIOGRAPHY; ELASTIC PROPERTIES; ELECTRIC STIMULI; LIQUID CHROMATOGRAPHY; MALES; MUSCULAR FUNCTION; RATS; RIBONUCLEIC ACIDS
Other Descriptors: COLLAGEN/BIOSYNTHESIS/CHEMISTRY/GENETICS; DIAPHRAGM/CHEMISTRY/INNERVATION/METABOLISM; GENE EXPRESSION/PHYSIOLOGY; AMINO ACIDS/METABOLISM; ANIMALS; AUTORADIOGRAPHY; BLOTTING, NORTHERN; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; ELASTICITY; ELECTRIC STIMULATION; EXTRACELLULAR SPACE/METABOLISM; MALE; MUSCLE CONTRACTION/PHYSIOLOGY; MUSCLE DENERVATION; PROCOLLAGEN/BIOSYNTHESIS/CHEMISTRY/GENETICS; RNA, MESSENGER/BIOSYNTHESIS; RATS; RATS, INBRED F344; SUPPORT, U.S. GOV'T, NON-P.H.S; SUPPORT, U.S. GOV'T, P.H.S; NASA DISCIPLINE MUSCULOSKELETAL; NON-NASA CENTER
Availability Source: Other Sources
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