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Record 98 of 323
Comparison of DNA fragmentation and color thresholding for objective quantitation of apoptotic cells
Author and Affiliation:
Plymale, D. R.(Tulane University School of Medicine, Molecular and Cellular Biology Program, New Orleans, LA 70112-2699, United States)
Ng Tang, D. S.
Fermin, C. D.
Lewis, D. E.
Martin, D. S.
Garry, R. F.
Abstract: Apoptosis is a process of cell death characterized by distinctive morphological changes and fragmentation of cellular DNA. Using video imaging and color thresholding techniques, we objectively quantitated the number of cultured CD4+ T-lymphoblastoid cells (HUT78 cells, RH9 subclone) displaying morphological signs of apoptosis before and after exposure to gamma-irradiation. The numbers of apoptotic cells measured by objective video imaging techniques were compared to numbers of apoptotic cells measured in the same samples by sensitive apoptotic assays that quantitate DNA fragmentation. DNA fragmentation assays gave consistently higher values compared with the video imaging assays that measured morphological changes associated with apoptosis. These results suggest that substantial DNA fragmentation can precede or occur in the absence of the morphological changes which are associated with apoptosis in gamma-irradiated RH9 cells.
Publication Date: Sep 01, 1995
Document ID:
20050000156
(Acquired Jan 06, 2005)
Subject Category: LIFE SCIENCES (GENERAL)
Document Type: Journal Article
Publication Information: Scanning microscopy (ISSN 0891-7035); Volume 9; 3; 833-42
Publisher Information: United States
Contract/Grant/Task Num: AI36211; AI43754; DE10862
Description: In English
Distribution Limits: Unclassified; Publicly available; Unlimited
Rights: Copyright
NASA Terms: APOPTOSIS; COLOR; DEOXYRIBONUCLEIC ACID; FRAGMENTATION; CULTURED CELLS; GAMMA RAYS; LYMPHOCYTES; MICROSCOPY; TUMORS
Other Descriptors: APOPTOSIS; DNA/METABOLISM; CD4-POSITIVE T-LYMPHOCYTES/PHYSIOLOGY/RADIATION EFFECTS; COLOR; COMPARATIVE STUDY; GAMMA RAYS; HUMAN; MICROSCOPY, VIDEO; SUPPORT, U.S. GOV'T, NON-P.H.S; SUPPORT, U.S. GOV'T, P.H.S; TUMOR CELLS, CULTURED; NASA DISCIPLINE NEUROSCIENCE; NON-NASA CENTER
Availability Source: Other Sources
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