NASA Logo

NTRS

NTRS - NASA Technical Reports Server

Back to Results
Isolation, characterization, and primary structure of rubredoxin from the photosynthetic bacterium, Heliobacillus mobilisRubredoxin is a small nonheme iron protein that serves as an electron carrier in bacterial systems. Rubredoxin has now been isolated and characterized from the strictly anaerobic phototroph, Heliobacillus mobilis. THe molecular mass (5671.3 Da from the amino acid sequence) was confirmed and partial formylation of the N-terminal methionyl residue was established by matrix-assisted laser desorption mass spectroscopy. The complete 52-amino-acid sequence was determined by a combination of N-terminal sequencing by Edman degradation and C-terminal sequencing by a novel method using carboxypeptidase treatment in conjunction with amino acid analysis and laser desorption time of flight mass spectrometry. The molar absorption coefficient of Hc. mobilis rubredoxin at 490 nm is 6.9 mM-1 cm-1 and the midpoint redox potential at pH 8.0 is -46 mV. The EPR spectrum of the oxidized form shows resonances at g = 9.66 and 4.30 due to a high-spin ferric iron. The amino acid sequence is homologous to those of rubredoxins from other species, in particular, the gram-positive bacteria, and the phototrophic green sulfur bacteria, and the evolutionary implications of this are discussed.
Document ID
20050000208
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
External Source(s)
Authors
Lee, W. Y.
(Arizona State University Temple 85287-1604, United States)
Brune, D. C.
LoBrutto, R.
Blankenship, R. E.
Date Acquired
August 22, 2013
Publication Date
April 1, 1995
Publication Information
Publication: Archives of biochemistry and biophysics
Volume: 318
Issue: 1
ISSN: 0003-9861
Subject Category
Life Sciences (General)
Distribution Limits
Public
Copyright
Other
Keywords
NASA Discipline Number 52-30
NASA Program Exobiology
Non-NASA Center
NASA Discipline Exobiology

Available Downloads

There are no available downloads for this record.
No Preview Available