NASA Logo, External Link
Facebook icon, External Link to NASA STI page on Facebook Twitter icon, External Link to NASA STI on Twitter YouTube icon, External Link to NASA STI Channel on YouTube RSS icon, External Link to New NASA STI RSS Feed AddThis share icon
 

Record Details

Record 7 of 1449
Cleavage of an amide bond by a ribozyme
Author and Affiliation:
Dai, X.(Scripps Research Institute, Department of Chemistry, La Jolla, CA 92037)
De Mesmaeker, A.
Joyce, G. F.
Miller, S. L. [Principal Investigator]
Abstract: A variant form of a group I ribozyme, optimized by in vitro evolution for its ability to catalyze magnesium-dependent phosphoester transfer reactions involving DNA substrates, also catalyzes the cleavage of an unactivated alkyl amide when that linkage is presented in the context of an oligodeoxynucleotide analog. Substrates containing an amide bond that joins either two DNA oligos, or a DNA oligo and a short peptide, are cleaved in a magnesium-dependent fashion to generate the expected products. The first-order rate constant, kcat, is 0.1 x 10(-5) min-1 to 1 x 10(-5) min-1 for the DNA-flanked substrates, which corresponds to a rate acceleration of more than 10(3) as compared with the uncatalyzed reaction.
Publication Date: Jan 13, 1995
Document ID:
20050000243
(Acquired Jan 06, 2005)
Subject Category: EXOBIOLOGY
Document Type: Journal Article
Publication Information: Science (ISSN 0036-8075); Volume 267; 5195; 237-40
Publisher Information: United States
Description: In English
Distribution Limits: Unclassified; Publicly available; Unlimited
Rights: Copyright
NASA Terms: AMIDES; CLEAVAGE; JOINTS (JUNCTIONS); POLYNUCLEOTIDES; RIBONUCLEIC ACIDS; EXOBIOLOGY; KINETICS; MAGNESIUM; POLYPEPTIDES; PROTOZOA
Other Descriptors: AMIDES/METABOLISM; OLIGODEOXYRIBONUCLEOTIDES/METABOLISM; RNA, CATALYTIC/METABOLISM; ANIMALS; BASE COMPOSITION; BASE SEQUENCE; KINETICS; MAGNESIUM/METABOLISM; MOLECULAR SEQUENCE DATA; OLIGOPEPTIDES/METABOLISM; SUPPORT, U.S. GOV'T, NON-P.H.S; TETRAHYMENA/ENZYMOLOGY; NASA DISCIPLINE EXOBIOLOGY; NON-NASA CENTER
Availability Source: Other Sources
› Back to Top
Find Similar Records
NASA Logo, External Link
NASA Official: Gerald Steeman
Site Curator: STI Program
Last Modified: August 23, 2011
Contact Us