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Noradrenergic lesioning with an anti-dopamine beta-hydroxylase immunotoxinSympathectomy has been achieved by a variety of methods but each has its limitations. These include lack of tissue specificity, incomplete lesioning, and the age range of susceptibility to the lesioning. To circumvent these drawbacks, an immunotoxin was constructed using a monoclonal antibody against the noradrenergic specific enzyme dopamine beta-hydroxylase (D beta H) coupled via a disulfide bond to saporin, a ribosomal inactivating protein. Three days after intravenous injection of the anti-D beta H immunotoxin (50 micrograms) into adult Sprague-Dawley rats, 66% of neurons in the superior cervical ganglia were chromatolytic. Superior cervical ganglia neurons were poisoned in 1 day old and 1 week old (86% of neurons) neonatal rats following subcutaneous injection of 3.75 and 15 micrograms, respectively. The anti-D beta H immunotoxin will be a useful tool in the study of the peripheral noradrenergic system in adult and neonatal animals.
Document ID
20050000275
Document Type
Reprint (Version printed in journal)
Authors
Picklo, M. J. (Vanderbilt University Nashville, TN 37232)
Wiley, R. G.
Lappi, D. A.
Robertson, D.
Date Acquired
August 22, 2013
Publication Date
December 15, 1994
Publication Information
Publication: Brain research
Volume: 666
Issue: 2
ISSN: 0006-8993
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: DK18811
CONTRACT_GRANT: HL44589
CONTRACT_GRANT: RR00095
Distribution Limits
Public
Copyright
Other
Keywords
Non-NASA Center
NASA Discipline Regulatory Physiology