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Metaphase yields from staphylococcal enterotoxin A stimulated peripheral blood lymphocytes of unirradiated and irradiated aged rhesus monkeysThe mitogen phytohemagglutinin (PHA) works well in both human and cynomolgus monkey (Macaca fascicularis) lymphocyte cultures to stimulate T cell proliferation. T cells from rhesus monkeys (Macaca mulatta) are less responsive than human cells, producing few metaphases when thousands are required, e.g. in biological dosimetry studies. We show that staphylococcal enterotoxin A (SEA), one of the most potent mitogens known, at a concentration of 0.5 microgram/ml stimulated peripheral lymphocytes to grow with a mitotic index (MI) averaging 0.13 metaphases/cell in old, irradiated rhesus macaques. This was significantly greater (p < 0.001) than that produced by PHA (MI < 0.01) in lymphocytes from the same animals. Whole blood was cultured for 96, 120 and 144 h for five irradiated individuals and for two controls. All cells cultured with SEA produced a high MI with a peak response at 120 h whereas the same cultures showed low MI for each PHA stimulated culture.
Document ID
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Hill, F. S.
(Lawrence Livermore National Laboratory, University of California Livermore 94551)
Cox, A. B.
Salmon, Y. L.
Cantu, A. O.
Lucas, J. N.
Date Acquired
August 22, 2013
Publication Date
October 1, 1994
Publication Information
Publication: International journal of radiation biology
Volume: 66
Issue: 4
ISSN: 0955-3002
Subject Category
Life Sciences (General)
Distribution Limits
Non-NASA Center
NASA Discipline Radiation Health

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