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Record 64 of 862
Decreased catecholamine secretion from the adrenal medullae of chronically diabetic BB-Wistar rats
Author and Affiliation:
Wilke, R. A.(Medical College of Wisconsin, Department of Pharmacology and Toxicology, Milwaukee 53226)
Riley, D. A.
Lelkes, P. I.
Hillard, C. J.
Abstract: Many humans with IDDM eventually lose the capacity to secrete epinephrine from their adrenal medullae. The mechanism for this pathological change is unknown. We hypothesized that this abnormality is attributable to neuropathic changes in the greater splanchnic nerves or in the chromaffin cells that they innervate. To study this hypothesis, we isolated rat adrenal glands, perfused them ex vivo, and measured the epinephrine content of the perfusate under various conditions of stimulation. We used transmural electrical stimulation (20-80 V, at 10 Hz) to induce epinephrine secretion indirectly by selectively activating residual splanchnic nerve terminals within the isolated glands. Under these conditions, epinephrine secretion was severely attenuated in glands from female BB-Wistar rats with diabetes of 4 mo duration compared with their age-matched, nondiabetic controls. These perfused diabetic adrenal medullae also demonstrated decreased catecholamine release in response to direct chromaffin cell depolarization with 20 mM K+, evidence that a functional alteration exists within the chromaffin cells themselves. Nonetheless, total catecholamine content of adrenal medullae from these diabetic rats was not significantly different from controls, indicating that the secretory defect was not simply attributable to a difference in the amount of catecholamines stored and available for release. Herein, we also provide histological evidence of degenerative changes within the cholinergic nerve terminals that innervate these glands.
Publication Date: Jun 01, 1993
Document ID:
20050000456
(Acquired Jan 06, 2005)
Subject Category: LIFE SCIENCES (GENERAL)
Document Type: Journal Article
Publication Information: Diabetes (ISSN 0012-1797); Volume 42; 6; 862-8
Publisher Information: United States
Contract/Grant/Task Num: R29-DA04800
Description: In English
Distribution Limits: Unclassified; Publicly available; Unlimited
Rights: Copyright
NASA Terms: ADRENAL GLAND; CATECHOLAMINE; DIABETES MELLITUS; METABOLIC DISEASES; RATS; SECRETIONS; ANALYSIS OF VARIANCE; CHRONIC CONDITIONS; FEMALES; NERVES
Other Descriptors: ADRENAL MEDULLA/INNERVATION/SECRETION; CATECHOLAMINES/SECRETION; DIABETES MELLITUS, TYPE I/METABOLISM; ANALYSIS OF VARIANCE; ANIMALS; CHRONIC DISEASE; DIABETIC NEUROPATHIES/METABOLISM; FEMALE; IN VITRO; RATS; RATS, INBRED BB; SPLANCHNIC NERVES/METABOLISM; SUPPORT, NON-U.S. GOV'T; SUPPORT, U.S. GOV'T, NON-P.H.S; SUPPORT, U.S. GOV'T, P.H.S; NASA DISCIPLINE MUSCULOSKELETAL; NON-NASA CENTER
Availability Source: Other Sources
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