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Temporal expression and immunogold localization of Plodia interpunctella granulosis virus structural proteinsMonospecific antisera were produced against four structural proteins (VP12, VP17, VP31, and granulin) of the Plodia interpunctella granulosis virus using polypeptides derived by sodium dodecyl sulfate-polyacrylamide gel electrophoresis or acid extraction. The antisera were shown to be specific on immunoblots of SDS-PAGE separated granulosis virus and were further used to detect structural proteins in infected fat body lysates. Immunoblots of fat body lysates from early stages of infection indicated that VP12, VP17, VP31, and granulin were expressed by 2.5 days post-infection. Immunogold labeling of the virus using the monospecific antisera and electron microscopy confirmed earlier reports that granulin is located in the protein matrix, V17 is an envelope protein, and VP31 is a capsid protein.
Document ID
20050000490
Document Type
Reprint (Version printed in journal)
Authors
Funk, C. J. (Kansas State University Manhattan 66506)
Consigli, R. A.
Spooner, B. S.
Date Acquired
August 22, 2013
Publication Date
April 1, 1993
Publication Information
Publication: Virus research
Volume: 28
Issue: 1
ISSN: 0168-1702
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: CA09418
Distribution Limits
Public
Copyright
Other
Keywords
Non-NASA Center
NASA Discipline Cell Biology