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Regional differences in lectin binding patterns of vestibular hair cellsSurface glycoconjugates of hair cells and supporting cells in the vestibular endorgans of the bullfrog were identified using biotinylated lectins with different carbohydrate specificities. Lectin binding in hair cells was consistent with the presence of glucose and mannose (CON A), galactose (RCA-I), N-acetylglucosamine (WGA), N-acetylgalactosamine (VVA), but not fucose (UEA-I) residues. Hair cells in the bullfrog sacculus, unlike those in the utriculus and semicircular canals, did not strain for N-acetylglucosamine (WGA) or N-acetylgalactosamine (VVA). By contrast, WGA and, to a lesser extent, VVA, differentially stained utricular and semicircular canal hair cells, labeling hair cells located in peripheral, but not central, regions. In mammals, WGA uniformly labeled Type I hair cells while labeling, as in the bullfrog, Type II hair cells only in peripheral regions. These regional variations were retained after enzymatic digestion. We conclude that vestibular hair cells differ in their surface glycoconjugates and that differences in lectin binding patterns can be used to identify hair cell types and to infer the epithelial origin of isolated vestibular hair cells.
Document ID
20050000516
Document Type
Reprint (Version printed in journal)
Authors
Baird, R. A.
(R.S. Dow Neurological Sciences Institute, Good Samaritan Hospital and Medical Center Portland, Oregon 97209)
Schuff, N. R.
Bancroft, J.
Date Acquired
August 22, 2013
Publication Date
February 1, 1993
Publication Information
Publication: Hearing research
Volume: 65
Issue: 2-Jan
ISSN: 0378-5955
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: DC-00355
Distribution Limits
Public
Copyright
Other
Keywords
NASA Discipline Neuroscience
Non-NASA Center
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