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Phylogeny of the ammonia-producing ruminal bacteria Peptostreptococcus anaerobius, Clostridium sticklandii, and Clostridium aminophilum sp. novIn previous studies, gram-positive bacteria which grew rapidly with peptides or an amino acid as the sole energy source were isolated from bovine rumina. Three isolates, strains C, FT (T = type strain), and SR, were considered to be ecologically important since they produced up to 20-fold more ammonia than other ammonia-producing ruminal bacteria. On the basis of phenotypic criteria, the taxonomic position of these new isolates was uncertain. In this study, the 16S rRNA sequences of these isolates and related bacteria were determined to establish the phylogenetic positions of the organisms. The sequences of strains C, FT, and SR and reference strains of Peptostreptococcus anaerobius, Clostridium sticklandii, Clostridium coccoides, Clostridium aminovalericum, Acetomaculum ruminis, Clostridium leptum, Clostridium lituseburense, Clostridium acidiurici, and Clostridium barkeri were determined by using a modified Sanger dideoxy chain termination method. Strain C, a large coccus purported to belong to the genus Peptostreptococcus, was closely related to P. anaerobius, with a level of sequence similarity of 99.6%. Strain SR, a heat-resistant, short, rod-shaped organism, was closely related to C. sticklandii, with a level of sequence similarity of 99.9%. However, strain FT, a heat-resistant, pleomorphic, rod-shaped organism, was only distantly related to some clostridial species and P. anaerobius. On the basis of the sequence data, it was clear that strain FT warranted designation as a separate species. The closest known relative of strain FT was C. coccoides (level of similarity, only 90.6%). Additional strains that are phenotypically similar to strain FT were isolated in this study.(ABSTRACT TRUNCATED AT 250 WORDS).
Document ID
20050000531
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Paster, B. J.
(Forsyth Dental Center Boston, Massachusetts 02115)
Russell, J. B.
Yang, C. M.
Chow, J. M.
Woese, C. R.
Tanner, R.
Date Acquired
August 22, 2013
Publication Date
January 1, 1993
Publication Information
Publication: International journal of systematic bacteriology
Volume: 43
Issue: 1
ISSN: 0020-7713
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: DE-04881
CONTRACT_GRANT: DE-08303
Distribution Limits
Public
Copyright
Other
Keywords
NASA Program Exobiology
Non-NASA Center
NASA Discipline Number 52-30
NASA Discipline Exobiology

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