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Record 32 of 7541
Dioctanoylglycerol stimulates accumulation of [methyl-14C]choline and its incorporation into acetylcholine and phosphatidylcholine in a human cholinergic neuroblastoma cell line
External Online Source: doi:10.1016/0006-8993(92)91203-Q
Author and Affiliation:
Slack, B. E.(Massachusetts Institute of Technology, Department of Brain and Cognitive Sciences, Cambridge 02139)
Richardson, U. I.
Nitsch, R. M.
Wurtman, R. J.
Abstract: Dioctanoylglycerol, a synthetic diacylglycerol, stimulated [14C]choline uptake in cultured human neuroblastoma (LA-N-2) cells. As this effect has not, to our knowledge, been reported before, it was of interest to characterize it in more detail. In the presence of 500 microM dioctanoylglycerol the levels of [14C]choline attained during a 2 hour labeling period were elevated by 78 +/- 12%, while [14C]acetylcholine and long fatty acyl chain [14C]phosphatidylcholine levels increased by 26 +/- 2% and 19 +/- 5%, respectively (mean +/- S.E.M.). Total (long chain plus dioctanoyl-) [14C]phosphatidylcholine was increased by 198 +/- 33%. Kinetic analysis showed that dioctanoylglycerol reduced the apparent Km for choline uptake to 56 +/- 9% of control (n = 4). The Vmax was not significantly altered. The stimulation of [14C]choline accumulation by dioctanoylglycerol was not dependent on protein kinase C activation; the effect was not mimicked by phorbol ester or by 1-oleoyl-2-acetylglycerol, and was not inhibited by the protein kinase C inhibitors H-7 or staurosporine, or by prolonged pretreatment with phorbol 12-myristate 13-acetate. The effect of dioctanoylglycerol was slightly (but not significantly) reduced by EGTA and strongly inhibited by the cell-permeant calcium chelator bis(o-aminophenoxy)-ethane-N,N,N',N'-tetraacetic acid, tetra(acetoxymethyl)ester. Although these results implicate elevated intracellular calcium in the response, dioctanoylglycerol did not increase phosphatidylinositol hydrolysis in LA-N-2 cells, and its effect was not inhibited by the diacylglycerol kinase inhibitor R 59 022 (which blocks the conversion of diacylglycerol to phosphatidic acid, a known stimulator of phosphatidylinositol hydrolysis).(ABSTRACT TRUNCATED AT 250 WORDS).
Publication Date: Jul 10, 1992
Document ID:
20050000643
(Acquired Jan 06, 2005)
Subject Category: LIFE SCIENCES (GENERAL)
Document Type: Journal Article
Publication Information: Brain research (ISSN 0006-8993); Volume 585; 1-2; 169-76
Publisher Information: Netherlands
Contract/Grant/Task Num: MH-28783
Description: In English
Distribution Limits: Unclassified; Publicly available; Unlimited
Rights: Copyright
NASA Terms: ACETYL COMPOUNDS; ACETYLCHOLINE; CHOLINE; CHOLINERGICS; METABOLISM; METHYL COMPOUNDS; NEOPLASMS; SYMPATHETIC NERVOUS SYSTEM; CULTURED CELLS; ENZYMES; OSMOSIS; TUMORS
Other Descriptors: ACETYLCHOLINE/BIOSYNTHESIS; CHOLINE/METABOLISM; DIGLYCERIDES/PHARMACOLOGY; NERVOUS SYSTEM NEOPLASMS/METABOLISM/PATHOLOGY; NEUROBLASTOMA/METABOLISM/PATHOLOGY; PARASYMPATHETIC NERVOUS SYSTEM; PHOSPHATIDYLCHOLINES/METABOLISM; HUMAN; OSMOLAR CONCENTRATION; PROTEIN KINASE C/METABOLISM; SUPPORT, NON-U.S. GOV'T; SUPPORT, U.S. GOV'T, P.H.S; TETRADECANOYLPHORBOL ACETATE/PHARMACOLOGY; TUMOR CELLS, CULTURED; NASA DISCIPLINE REGULATORY PHYSIOLOGY; NON-NASA CENTER
Availability Source: Other Sources
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