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Production and characterization of monoclonal antibodies to budgerigar fledgling disease virus major capsid protein VPEleven hybridoma cell lines producing monoclonal antibodies (MAbs) against intact budgerigar fledgling disease (BFD) virions were produced and characterized. These antibodies were selected for their ability to react with BFD virions in an enzyme-linked immunosorbent assay. Each of these antibodies was reactive in the immunofluorescent detection of BFD virus-infected cells. These antibodies immunoprecipitated intact virions and specifically recognized the major capsid protein, VP1, of the dissociated virion. The MAbs were found to preferentially recognize native BFD virus capsid protein when compared with denatured virus protein. These MAbs were capable of detecting BFD virus protein in chicken embryonated cell-culture lysates by dot-blot analysis.
Document ID
20050000656
Document Type
Reprint (Version printed in journal)
Authors
Fattaey, A. (Kansas State University Manhattan 66506)
Lenz, L.
Consigli, R. A.
Spooner, B. S.
Date Acquired
August 22, 2013
Publication Date
July 1, 1992
Publication Information
Publication: Avian diseases
Volume: 36
Issue: 3
ISSN: 0005-2086
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: CA07319
Distribution Limits
Public
Copyright
Other
Keywords
Non-NASA Center
NASA Discipline Cell Biology