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Indirect myosin immunocytochemistry for the identification of fibre types in equine skeletal muscleThe histochemical ATPase method for muscle fibre typing was first described by Brooke and Kaiser in 1970. However, problems have been found with the subdivision of type II fibres using this technique. To determine whether indirect myosin immunocytochemistry using anti-slow (5-4D), anti-fast (1A10) and anti-fast red (5-2B) monoclonal antibodies with cross reactivity for type I, II and IIa fibres, respectively, in a number of species, could identify three fibre types in equine skeletal muscle, data on fibre type composition and fibre size obtained using the two different techniques were compared. Results indicate that different myosin heavy chains can coexist in single equine muscle fibres. Type I and type II fibres were identified by immunocytochemistry, but subdivision of type II fibres was not possible. Although the percentage of type I and type II fibres was not significantly different for the two techniques, a few fibres reacted with both the 1A10 and 5-4D antibodies.
Document ID
20050000657
Document Type
Reprint (Version printed in journal)
Authors
Sinha, A. K. (University of Sydney New South Wales, Australia)
Rose, R. J.
Pozgaj, I.
Hoh, J. F.
Date Acquired
August 22, 2013
Publication Date
July 1, 1992
Publication Information
Publication: Research in veterinary science
Volume: 53
Issue: 1
ISSN: 0034-5288
Subject Category
Life Sciences (General)
Distribution Limits
Public
Copyright
Other
Keywords
NASA Discipline Musculoskeletal
Non-NASA Center