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Extracellular vesicles of calcifying turkey leg tendon characterized by immunocytochemistry and high voltage electron microscopic tomography and 3-D graphic image reconstructionTo gain insight into the structure and possible function of extracellular vesicles in certain calcifying vertebrate tissues, normally mineralizing leg tendons from the domestic turkey, Meleagris gallopavo, have been studied in two separate investigations, one concerning the electron microscopic immunolocalization of the 66 kDa phosphoprotein, osteopontin, and the other detailing the organization and distribution of mineral crystals associated with the vesicles as determined by high voltage microscopic tomography and 3-D graphic image reconstruction. Immunolabeling shows that osteopontin is related to extracellular vesicles of the tendon in the sense that its initial presence appears coincident with the development of mineral associated with the vesicle loci. By high voltage electron microscopy and 3-D imaging techniques, mineral crystals are found to consist of small irregularly shaped particles somewhat randomly oriented throughout individual vesicles sites. Their appearance is different from that found for the mineral observed within calcifying tendon collagen, and their 3-D disposition is not regularly ordered. Possible spatial and temporal relationships of vesicles, osteopontin, mineral, and collagen are being examined further by these approaches.
Document ID
20050000708
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Landis, W. J.
(Harvard Medical School Boston, MA 02115)
Hodgens, K. J.
McKee, M. D.
Nanci, A.
Song, M. J.
Kiyonaga, S.
Arena, J.
McEwen, B.
Date Acquired
August 22, 2013
Publication Date
May 1, 1992
Publication Information
Publication: Bone and mineral
Volume: 17
Issue: 2
ISSN: 0169-6009
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: GM40165
CONTRACT_GRANT: AR34078
CONTRACT_GRANT: AR34081
Distribution Limits
Public
Copyright
Other
Keywords
NASA Discipline Musculoskeletal
Non-NASA Center

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