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Crystal structure of an Okazaki fragment at 2-A resolutionIn DNA replication, Okazaki fragments are formed as double-stranded intermediates during synthesis of the lagging strand. They are composed of the growing DNA strand primed by RNA and the template strand. The DNA oligonucleotide d(GGGTATACGC) and the chimeric RNA-DNA oligonucleotide r(GCG)d(TATACCC) were combined to form a synthetic Okazaki fragment and its three-dimensional structure was determined by x-ray crystallography. The fragment adopts an overall A-type conformation with 11 residues per turn. Although the base-pair geometry, particularly in the central TATA part, is distorted, there is no evidence for a transition from the A- to the B-type conformation at the junction between RNA.DNA hybrid and DNA duplex. The RNA trimer may, therefore, lock the complete fragment in an A-type conformation.
Document ID
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Egli, M.
(Massachusetts Institute of Technology Cambridge 02139)
Usman, N.
Zhang, S. G.
Rich, A.
Date Acquired
August 22, 2013
Publication Date
January 15, 1992
Publication Information
Publication: Proceedings of the National Academy of Sciences of the United States of America
Volume: 89
Issue: 2
ISSN: 0027-8424
Subject Category
Distribution Limits
Non-NASA Center
NASA Discipline Exobiology

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