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Record Details

Record 28 of 658
Generation of avian cells resembling osteoclasts from mononuclear phagocytes
Author and Affiliation:
Alvarez, J. I.(Jewish Hospital, Washington University Medical Center, Department of Pathology and Laboratory Medicine, St. Louis, Missouri 63110)
Teitelbaum, S. L.
Blair, H. C.
Greenfield, E. M.
Athanasou, N. A.
Ross, F. P.
Abstract: Several lines of indirect evidence suggest that a monocyte family precursor gives rise to the osteoclast, although this hypothesis is controversial. Starting with a uniform population of nonspecific esterase positive, tartrate-sensitive, acid phosphatase-producing, mannose receptor-bearing mononuclear cells, prepared from dispersed marrow of calcium-deprived laying hens by cell density separation and selective cellular adherence, we generated multinucleated cells in vitro. When cultured with devitalized bone, these cells show, by electron microscopy, the characteristic osteoclast morphology in that they are mitochondria-rich, multinucleated, and, most importantly, develop characteristic ruffled membranes at the matrix attachment site. Moreover, as documented by scanning electron microscopy, these cells pit bone slices in a manner identical to freshly isolated osteoclasts. In addition, isoenzymes of acid phosphatase from generated osteoclasts, separated by 7.5% polyacrylamide gel electrophoresis at pH 4, are identical to those of mature osteoclasts in migration pattern and tartrate resistance, although the precursor cells from which the osteoclasts are generated produce an entirely different isoenzyme, which is tartrate-sensitive and migrates less rapidly at pH 4. The fused cells also exhibit a cAMP response to prostaglandin E2. Therefore, osteoclast-like cells can be derived by in vitro culture of a marrow-derived monocyte cell population.
Publication Date: May 01, 1991
Document ID:
20050000849
(Acquired Jan 06, 2005)
Subject Category: AEROSPACE MEDICINE
Document Type: Journal Article
Publication Information: Endocrinology; p. 2324-35; (ISSN 0013-7227); Volume 128; 5
Publisher Information: United States
Contract/Grant/Task Num: AR-32788
Description: In English
Distribution Limits: Unclassified; Publicly available; Unlimited
Rights: Copyright
NASA Terms: BIRDS; BONE DEMINERALIZATION; CHICKENS; LEUKOCYTES; BONE MARROW; CELLS (BIOLOGY); CULTURED CELLS; CYCLIC AMP; ELECTRON MICROSCOPY; METABOLISM; MONOCYTES
Other Descriptors: CHICKENS/PHYSIOLOGY; OSTEOCLASTS/METABOLISM/PHYSIOLOGY/ULTRASTRUCTURE; PHAGOCYTES/PHYSIOLOGY; ANIMALS; BONE MARROW CELLS; CELL FUSION; CELL LINE; CYCLIC AMP/BIOSYNTHESIS; HISTOCYTOCHEMISTRY; MICROSCOPY, ELECTRON; MONOCYTES/METABOLISM; SUPPORT, NON-U.S. GOV'T; SUPPORT, U.S. GOV'T, NON-P.H.S; SUPPORT, U.S. GOV'T, P.H.S; NASA DISCIPLINE MUSCULOSKELETAL; NON-NASA CENTER
Availability Source: Other Sources
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