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Marked and variable inhibition by chemical fixation of cytochrome oxidase and succinate dehydrogenase in single motoneuronsThe effect of tissue fixation on succinate dehydrogenase and cytochrome oxidase activity in single motoneurons of the rat was demonstrated using a computer image processing system. Inhibition of enzyme activity by chemical fixation was variable, with some motoneurons being affected more than others. It was concluded that quantification of enzymatic activity in chemically fixed tissue provides an imprecise estimate of enzyme activities found in fresh-frozen tissues.
Document ID
20050000907
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Chalmers, G. R.
(University of California Los Angeles 90024)
Edgerton, V. R.
Date Acquired
August 22, 2013
Publication Date
June 1, 1989
Publication Information
Publication: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
Volume: 37
Issue: 6
ISSN: 0022-1554
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: NS-16333
Distribution Limits
Public
Copyright
Other
Keywords
NASA Discipline Musculoskeletal
Non-NASA Center

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