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Antigen presentation by non-immune B-cell hybridoma clones: presentation of synthetic antigenic sites reveals clones that exhibit no specificity and clones that present only one epitopeRecently, we reported the preparation and antigen-presenting properties of hybridoma B-cell clones obtained after fusing non-secreting, non-antigen presenting Balb/c 653-myeloma cells with non-immune SJL spleen cells. It was found that antigen presentation at the clonal level can be specific or non-specific, depending on the particular B-cell clone. In the present work, one specific and one general presenter B-cell clones were tested for their epitope presentation ability to SJL T-cells that were specific to lysozyme or myoglobin. B-cell clone A1G12, a general presenter which presented both lysozyme and myoglobin to their respective T-cell lines, was found to present all five myoglobin epitopes while clone A1L16, a lysozyme specific presenter presented only one of the three epitopes of lysozyme. The latter reveals a hitherto unknown submolecular specificity (to a given epitope within a protein) for antigen presenting cells at the clonal level. Therefore, the specificity of T-cell recognition does not only derive from the T-cell but may also be dependent on the epitope specificity of the antigen-presenting B-cell.
Document ID
20050025664
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Cohly, H. H.
(NASA Johnson Space Center Houston, TX United States)
Morrison, D. R.
Atassi, M. Z.
Date Acquired
August 22, 2013
Publication Date
October 1, 1989
Publication Information
Publication: Immunological investigations
Volume: 18
Issue: 8
ISSN: 0882-0139
Subject Category
Life Sciences (General)
Distribution Limits
Public
Copyright
Other

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