NASA Logo

NTRS

NTRS - NASA Technical Reports Server

Back to Results
Genome-wide transcriptional analysis of flagellar regeneration in Chlamydomonas reinhardtii identifies orthologs of ciliary disease genesThe important role that cilia and flagella play in human disease creates an urgent need to identify genes involved in ciliary assembly and function. The strong and specific induction of flagellar-coding genes during flagellar regeneration in Chlamydomonas reinhardtii suggests that transcriptional profiling of such cells would reveal new flagella-related genes. We have conducted a genome-wide analysis of RNA transcript levels during flagellar regeneration in Chlamydomonas by using maskless photolithography method-produced DNA oligonucleotide microarrays with unique probe sequences for all exons of the 19,803 predicted genes. This analysis represents previously uncharacterized whole-genome transcriptional activity profiling study in this important model organism. Analysis of strongly induced genes reveals a large set of known flagellar components and also identifies a number of important disease-related proteins as being involved with cilia and flagella, including the zebrafish polycystic kidney genes Qilin, Reptin, and Pontin, as well as the testis-expressed tubby-like protein TULP2.
Document ID
20050164249
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
External Source(s)
Authors
Stolc, Viktor
(NASA Ames Research Center Moffett Field, CA United States)
Samanta, Manoj Pratim
Tongprasit, Waraporn
Marshall, Wallace F.
Date Acquired
August 23, 2013
Publication Date
March 8, 2005
Publication Information
Publication: Proceedings of the National Academy of Sciences of the United States of America
Volume: 102
Issue: 10
ISSN: 0027-8424
Subject Category
Life Sciences (General)
Distribution Limits
Public
Copyright
Other

Available Downloads

There are no available downloads for this record.
No Preview Available