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Flow of Fluid and Particle Assemblages in Rotating SystemsNASA-designed bioreactors have been highly successful in growing three-dimensional tissue structures in a low shear environment both on earth and in space. The goal of the present study is to characterize the fluid flow environment within the HFB-S bioreactor and determine the spatial distribution of particles that mimic cellular tissue structures. The results will be used to obtain optimal operating conditions of rotation rates and media perfusehnfuse rates which are required for cell culture growth protocols. Two types of experiments have been performed so far. First, we have performed laser florescent dye visualization of the perfusion loop to determine the mixing times within the chamber. The second type of experiments involved particles which represent cellular tissue to determine the spatial distribution with the chamber. From these experiments we established that mixing times were largely dependant on the speed ratio and sign of the difference between the spinner and the dome. The shortest mixing times occurred when the spinner rotates faster than the dome and longest mixing times occurs with no relative motion between the dome and spinner. Also, we have determined the spatial and temporal distribution of particle assemblages within the chamber.
Document ID
20050205654
Acquisition Source
Glenn Research Center
Document Type
Conference Paper
Authors
Kizito, John
(National Center for Microgravity Research on Fluids and Combustion Cleveland, OH, United States)
Hiltner, David
(National Center for Microgravity Research on Fluids and Combustion Cleveland, OH, United States)
Niederhaus, Charles
(NASA Glenn Research Center Cleveland, OH, United States)
Kleis, Stanley
(Houston Univ. TX, United States)
Hudson, Ed
(NASA Johnson Space Center Houston, TX, United States)
Gonda, Steve
(NASA Johnson Space Center Houston, TX, United States)
Date Acquired
August 23, 2013
Publication Date
January 1, 2004
Subject Category
Space Processing
Meeting Information
Meeting: 2004 NASA Cell Science Conference
Location: Palo Alto, CA
Country: United States
Start Date: February 26, 2004
End Date: February 28, 2004
Funding Number(s)
OTHER: 22-400-21-60-01
Distribution Limits
Public
Copyright
Work of the US Gov. Public Use Permitted.

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