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Measurable characteristics of lysozyme crystal growthThe behavior of protein crystal growth is estimated from measurements performed at both the microscopic and molecular levels. In the absence of solutal flow, it was determined that a model that balances the macromolecular flux toward the crystal surface with the flux of the crystal surface well characterizes crystal growth observed using microscopic methods. Namely, it was determined that the model provides accurate estimates for the crystal-growth velocities upon evaluation of crystal-growth measurements obtained in time. Growth velocities thus determined as a function of solution supersaturation were further interpreted using established deterministic models. From analyses of crystal-growth velocities, it was found that the mode of crystal growth varies with respect to increasing solution supersaturation, possibly owing to kinetic roughening. To verify further the hypothesis of kinetic roughening, crystal growth at the molecular level was examined using atomic force microscopy (AFM). From the AFM measurements, it was found that the magnitude of surface-height fluctuations, h(x), increases with increasing solution supersaturation. In contrast, the estimated characteristic length, xi, decreases rapidly upon increasing solution supersaturation. It was conjectured that the magnitude of both h(x) and xi could possibly determine the mode of crystal growth. Although the data precede any exact theory, the non-critical divergence of h(x) and xi with respect to increasing solution supersaturation was nevertheless preliminarily established. Moreover, approximate models to account for behavior of both h(x) and xi are also presented.
Document ID
Document Type
Reprint (Version printed in journal)
Gorti, Sridhar
(NASA Headquarters Washington, DC United States)
Forsythe, Elizabeth L.
Pusey, Marc L.
Date Acquired
August 23, 2013
Publication Date
June 1, 2005
Publication Information
Publication: Acta crystallographica. Section D, Biological crystallography
Volume: 61
Issue: Pt 6
ISSN: 0907-4449
Subject Category
Life Sciences (General)
Distribution Limits

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