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Record 3 of 3526
Complex regulation of Arabidopsis AGR1/PIN2-mediated root gravitropic response and basipetal auxin transport by cantharidin-sensitive protein phosphatases
External Online Source: doi:10.1111/j.1365-313X.2005.02369.x
Author and Affiliation:
Shin, Heungsop(Samuel Roberts Noble Foundation, Plant Biology Division, 2510 Sam Noble Parkway, Ardmore, OK 73401, United States)
Shin, Hwa-Soo
Guo, Zibiao
Blancaflor, Elison B.
Masson, Patrick H.
Chen, Rujin
Abstract: Polar auxin transport, mediated by two distinct plasma membrane-localized auxin influx and efflux carrier proteins/complexes, plays an important role in many plant growth and developmental processes including tropic responses to gravity and light, development of lateral roots and patterning in embryogenesis. We have previously shown that the Arabidopsis AGRAVITROPIC 1/PIN2 gene encodes an auxin efflux component regulating root gravitropism and basipetal auxin transport. However, the regulatory mechanism underlying the function of AGR1/PIN2 is largely unknown. Recently, protein phosphorylation and dephosphorylation mediated by protein kinases and phosphatases, respectively, have been implicated in regulating polar auxin transport and root gravitropism. Here, we examined the effects of chemical inhibitors of protein phosphatases on root gravitropism and basipetal auxin transport, as well as the expression pattern of AGR1/PIN2 gene and the localization of AGR1/PIN2 protein. We also examined the effects of inhibitors of vesicle trafficking and protein kinases. Our data suggest that protein phosphatases, sensitive to cantharidin and okadaic acid, are likely involved in regulating AGR1/PIN2-mediated root basipetal auxin transport and gravitropism, as well as auxin response in the root central elongation zone (CEZ). BFA-sensitive vesicle trafficking may be required for the cycling of AGR1/PIN2 between plasma membrane and the BFA compartment, but not for the AGR1/PIN2-mediated root basipetal auxin transport and auxin response in CEZ cells.
Publication Date: Apr 01, 2005
Document ID:
20050207042
(Acquired Sep 01, 2005)
Subject Category: LIFE SCIENCES (GENERAL)
Document Type: Journal Article
Publication Information: The Plant journal : for cell and molecular biology (ISSN 0960-7412); Volume 42; 2; 188-200
Publisher Information: United Kingdom
Description: In English
Distribution Limits: Unclassified; Publicly available; Unlimited
Rights: Copyright
NASA Terms: AUXINS; GRAVITROPISM; HORMONES; PHOSPHATASES; PLANT ROOTS; PLANTS (BOTANY); PROTEINS; SENSITIVITY; GENE EXPRESSION REGULATION; METABOLISM; MUTATIONS; TIME DEPENDENCE
Other Descriptors: ARABIDOPSIS/GROWTH & DEVELOPMENT/METABOLISM/PHYSIOLOGY; ARABIDOPSIS PROTEINS/METABOLISM/PHYSIOLOGY; AUXINS/METABOLISM; GRAVITROPISM/PHYSIOLOGY; MEMBRANE TRANSPORT PROTEINS/METABOLISM/PHYSIOLOGY; PHOSPHOPROTEIN PHOSPHATASE/METABOLISM; PLANT ROOTS/GROWTH & DEVELOPMENT/METABOLISM/PHYSIOLOGY; BIOLOGICAL TRANSPORT/PHYSIOLOGY; CANTHARIDIN/METABOLISM/PHARMACOLOGY; GENE EXPRESSION REGULATION, PLANT/PHYSIOLOGY; MUTATION; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S; TIME FACTORS; NASA DISCIPLINE PLANT BIOLOGY; NON-NASA CENTER
Availability Source: Other Sources
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