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Space Station Biological Research Project (SSBRP) Cell Culture Unit (CCU) and incubator for International Space Station (ISS) cell culture experimentsThe CCU and Incubator are habitats under development by SSBRP for gravitational biology research on ISS. They will accommodate multiple specimen types and reside in either Habitat Holding Racks, or the Centrifuge Rotor, which provides selectable gravity levels of up to 2 g. The CCU can support multiple Cell Specimen Chambers, CSCs (18, 9 or 6 CSCs; 3, 10 or 30 mL in volume, respectively). CSCs are temperature controlled from 4-39 degrees C, with heat shock to 45 degrees C. CCU provides automated nutrient supply, magnetic stirring, pH/O2 monitoring, gas supply, specimen lighting, and video microscopy. Sixty sample containers holding up to 2 mL each, stored at 4-39 degrees C, are available for automated cell sampling, subculture, and injection of additives and fixatives. CSCs, sample containers, and fresh/spent media bags are crew-replaceable for long-term experiments. The Incubator provides a 4-45 degrees C controlled environment for life science experiments or storage of experimental reagents. Specimen containers and experiment unique equipment are experimenter-provided. The Specimen Chamber exchanges air with ISS cabin and has 18.8 liters of usable volume that can accommodate six trays and the following instrumentation: five relocatable thermometers, two 60 W power outlets, four analog ports, and one each relative humidity sensor, video port, ethernet port and digital input/output port.
Document ID
20050218221
Document Type
Reprint (Version printed in journal)
Authors
Vandendriesche, Donald (NASA Ames Research Center Moffett Field, CA United States)
Parrish, Joseph
Kirven-Brooks, Melissa
Fahlen, Thomas
Larenas, Patricia
Havens, Cindy
Nakamura, Gail
Sun, Liping
Krebs, Chris
de Luis, Javier
Vunjak-Novakovic, Gordana
Searby, Nancy D.
Date Acquired
August 23, 2013
Publication Date
March 1, 2004
Publication Information
Publication: Journal of gravitational physiology : a journal of the International Society for Gravitational Physiology
Volume: 11
Issue: 1
ISSN: 1077-9248
Subject Category
Aerospace Medicine
Distribution Limits
Public
Copyright
Other
Keywords
NASA Center ARC
NASA Discipline Cell Biology