NASA Logo, External Link
Facebook icon, External Link to NASA STI page on Facebook Twitter icon, External Link to NASA STI on Twitter YouTube icon, External Link to NASA STI Channel on YouTube RSS icon, External Link to New NASA STI RSS Feed AddThis share icon
 

Record Details

Record 3 of 2020
Three-dimensional characterization of tethered microspheres by total internal reflection fluorescence microscopy
External Online Source: doi:10.1529/biophysj.105.061242
Author and Affiliation:
Blumberg, Seth(University of Michigan, Department of Physics, Biophysics Research Division, Randall Laboratory, Ann Arbor, 48109-1120, United States)
Gajraj, Arivalagan
Pennington, Matthew W.
Meiners, Jens-Christian
Abstract: Tethered particle microscopy is a powerful tool to study the dynamics of DNA molecules and DNA-protein complexes in single-molecule experiments. We demonstrate that stroboscopic total internal reflection microscopy can be used to characterize the three-dimensional spatiotemporal motion of DNA-tethered particles. By calculating characteristic measures such as symmetry and time constants of the motion, well-formed tethers can be distinguished from defective ones for which the motion is dominated by aberrant surface effects. This improves the reliability of measurements on tether dynamics. For instance, in observations of protein-mediated DNA looping, loop formation is distinguished from adsorption and other nonspecific events.
Publication Date: Aug 01, 2005
Document ID:
20050232386
(Acquired Nov 01, 2005)
Subject Category: LIFE SCIENCES (GENERAL)
Document Type: Journal Article
Publication Information: Biophysical journal; p. 1272-81; (ISSN 0006-3495); Volume 89; 2
Publisher Information: United States
Contract/Grant/Task Num: GM007863; GM65934
Financial Sponsor: NASA Glenn Research Center; Cleveland, OH United States
Description: In English
Distribution Limits: Unclassified; Publicly available; Unlimited
Rights: Copyright
NASA Terms: COMPUTER TECHNIQUES; DEOXYRIBONUCLEIC ACID; FLUORESCENCE; IMAGE ANALYSIS; IMAGE ENHANCEMENT; IMAGING TECHNIQUES; MICROPARTICLES; MICROSCOPY; TETHERING; MOTION; NUCLEIC ACIDS
Other Descriptors: DNA/ANALYSIS/CHEMISTRY/ULTRASTRUCTURE; IMAGE ENHANCEMENT/INSTRUMENTATION/METHODS; IMAGE INTERPRETATION, COMPUTER-ASSISTED/INSTRUMENTATION/METHODS; IMAGING, THREE-DIMENSIONAL/INSTRUMENTATION/METHODS; MICROSCOPY, FLUORESCENCE/INSTRUMENTATION/METHODS; STROBOSCOPY/INSTRUMENTATION/METHODS; MICROSPHERES; MOTION; NUCLEIC ACID CONFORMATION; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S; RESEARCH SUPPORT, U.S. GOV'T, P.H.S; NASA DISCIPLINE CELL BIOLOGY; NON-NASA CENTER; EVALUATION STUDIES
Availability Source: Other Sources
› Back to Top
NASA Logo, External Link
NASA Official: Gerald Steeman
Site Curator: STI Program
Last Modified: August 23, 2011
Contact Us