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Record 4 of 120
Three-Dimensionally Engineered Normal Human Broncho-epithelial Tissue-Like Assemblies: Target Tissues for Human Respiratory Viral Infections
Author and Affiliation:
Goodwin, T. J.(NASA Johnson Space Center, Houston, TX, United States)
McCarthy, M.(NASA Johnson Space Center, Houston, TX, United States)
Lin, Y-H(Wyeth Research, Pearl River, NY, United States)
Abstract: In vitro three-dimensional (3D) human broncho-epithelial (HBE) tissue-like assemblies (3D HBE TLAs) from this point forward referred to as TLAs were engineered in Rotating Wall Vessel (RWV) technology to mimic the characteristics of in vivo tissues thus providing a tool to study human respiratory viruses and host cell interactions. The TLAs were bioengineered onto collagen-coated cyclodextran microcarriers using primary human mesenchymal bronchial-tracheal cells (HBTC) as the foundation matrix and an adult human bronchial epithelial immortalized cell line (BEAS-2B) as the overlying component. The resulting TLAs share significant characteristics with in vivo human respiratory epithelium including polarization, tight junctions, desmosomes, and microvilli. The presence of tissue-like differentiation markers including villin, keratins, and specific lung epithelium markers, as well as the production of tissue mucin, further confirm these TLAs differentiated into tissues functionally similar to in vivo tissues. Increasing virus titers for human respiratory syncytial virus (wtRSVA2) and parainfluenza virus type 3 (wtPIV3 JS) and the detection of membrane bound glycoproteins over time confirm productive infections with both viruses. Therefore, TLAs mimic aspects of the human respiratory epithelium and provide a unique capability to study the interactions of respiratory viruses and their primary target tissue independent of the host's immune system.
Publication Date: Jan 01, 2006
Document ID:
20060013131
(Acquired May 17, 2006)
Subject Category: LIFE SCIENCES (GENERAL)
Document Type: Conference Paper
Meeting Information: 2006 Society for Industrial Microbiology Annual Meeting; 30 Jul. - 3 aug. 2006; Baltimore, MD; United States
Financial Sponsor: NASA Johnson Space Center; Houston, TX, United States
Organization Source: NASA Johnson Space Center; Houston, TX, United States
Description: 2p; In English
Distribution Limits: Unclassified; Publicly available; Unlimited
Rights: No Copyright
NASA Terms: BRONCHI; EPITHELIUM; VIRAL DISEASES; TISSUES (BIOLOGY); IN VITRO METHODS AND TESTS; RESPIRATORY SYSTEM; KERATINS; LUNGS; COLLAGENS; ADULTS; BIOMARKERS; IMMUNE SYSTEMS; CELLS (BIOLOGY)
Availability Source: Other Sources
Availability Notes: Abstract Only
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