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Soluble Protein Analysis using a Compact Bench-top Flow CytometerFuture space exploration missions will require analytical technology capable of providing both autonomous medical care to the crew and investigative capabilities to researchers. While several promising candidate technologies exist for further development, flow cytometry is an attractive technology as it offers both crew health (blood cell count, leukocyte differential, etc.) and a wide array of biochemistry and immunology assays. research settings, the application of this technique to soluble protein analysis is also possible. Proteomic beads using fluorescent dyes for optical encoding were used to monitor six cytokines simultaneously in cell medium of cell cultures in stationary and rotating cell culture systems. The results of this work demonstrate that a compact flow cytometer, such as a system proposed for space flight, can detect a variety of soluble proteins for crew health and biotechnology experiments during long-term missions.
Document ID
20060013501
Document Type
Conference Paper
Authors
Pappas, Dimitri (Wyle Labs., Inc. Houston, TX, United States)
Kao, Shib-Hsin (Equal Access to Software and Information (EASI) Lake Forest, CA, United States)
Cyr, Johnathan (Universities Space Research Association Houston, TX, United States)
Date Acquired
August 23, 2013
Publication Date
January 1, 2004
Subject Category
Aerospace Medicine
Report/Patent Number
W04-009
Funding Number(s)
CONTRACT_GRANT: NAS9-02078
Distribution Limits
Public
Copyright
Work of the US Gov. Public Use Permitted.