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Microgravity and Signaling Molecules in Rat Osteoblasts: Downstream of Receptor Tyrosine Kinase, G-Protein-Coupled Receptor, and Small GTP-Binding ProteinsRat osteoblasts were cultured for 4 and 5 days aboard Space Shuttle and solubilized on board. The mRNA levels of the post-receptor signaling molecules were analyzed by quantitative RT-PCR. The G-protein alpha subunit G(alpha)q mRNA levels were elevated 3-fold by microgravity. G(alpha)q stimulates PLC(beta), and then PKC. PKC(delta) and PKC(theta) mRNA levels were increased 2- to 5-fold by microgravity The mRNA levels of SOS and Ras GRF were increased 4 to 5-fold by microgravity, while Ras GAP was not altered. Spaceflight-induced bone loss might be attributed to microgravity modulation of the signaling pathway in osteoblasts.
Document ID
20060054320
Acquisition Source
Goddard Space Flight Center
Document Type
Reprint (Version printed in journal)
Authors
Kumel, Yasuhiro
(Tokyo Medical and Dental Univ. Japan)
Shimokawa, Hitoyata
(Tokyo Medical and Dental Univ. Japan)
Morita, Sadao
(Tokyo Medical and Dental Univ. Japan)
Katano, Hisako
(Tokyo Univ. Japan)
Akiyama, Hideo
(Toray Research Center, Inc. Tokyo, Japan)
Hirano, Masahiko
(Toray Research Center, Inc. Tokyo, Japan)
Ohya, Keiichi
(Tokyo Medical and Dental Univ. Japan)
Sams, Clarence F.
(NASA Johnson Space Center Houston, TX, United States)
Whitson, Peggy A.
(NASA Johnson Space Center Houston, TX, United States)
Date Acquired
August 23, 2013
Publication Date
July 1, 2005
Publication Information
Publication: Journal of Gravitational Physiology, Volume 12, Number 1
Subject Category
Life Sciences (General)
Distribution Limits
Public
Copyright
Other

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