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Alterations in Vasoreactivity of Femoral Artery Induced by Hindlimb Unweighting are Related to the Changes of Contractile Protein in RatsResponses of endothelium removed femoral arterial rings to vasoactive compounds were examined in vitro, and the expression of Myosin and Actin of femoral artery were observed by Western Blotting and Immunohistochemistry in hndlimb unweighting rats and control rats. The results showed that contractile responses of femoral arterial rings evoked by Phenylephrine, Endothelin-1, Vasopressin, KCl, Ca(2+) and Ca(2+) ionophore A23187 were decreased in hindlimb unweighting rats as compared with that of controls. But vasoddatory responses induced by SNPand cGMP were not different between groups. No significant differences have been found in expressions of Calponin, Myosin, Actin, and the ratio of MHC SM1/SM2 between the two groups, but expression of alpha-SM-Actin decreased in hindlimb unweighting rats. The data indicated that the diminished contractile responsiveness probably result from altered contractile apparatus, especially the contractile proteins.
Document ID
20060054322
Acquisition Source
Goddard Space Flight Center
Document Type
Reprint (Version printed in journal)
Authors
Ma, Jin
(Fourth Military Medical Univ. Xi'an, China)
Ren, Xinling
(Xijing Hospital Xian, China)
Meng, Qinjun
(Fourth Military Medical Univ. Xi'an, China)
Zhang, Lifan
(Fourth Military Medical Univ. Xi'an, China)
Purdy, Ralph E.
(California Univ. Irvine, CA, United States)
Date Acquired
August 23, 2013
Publication Date
July 1, 2005
Publication Information
Publication: Journal of Gravitational Physiology, Volume 12, Number 1
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: 30170355
CONTRACT_GRANT: NAG9-1149
Distribution Limits
Public
Copyright
Other

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