NASA Logo

NTRS

NTRS - NASA Technical Reports Server

Back to Results
Trichococcus Patagoniensis sp. nov., a Facultative Anaerobe that grows at -5 C, Isolated from Penguin Guano in Chilean PatagoniaA novel, extremely psychrotolerant, facultative anaerobe, strain PmagGl(sup T), was isolated from guano of Magellanic penguins (Spheniscus magellanicus) collected in Chilean Patagonia. Gram-variable, motile cocci with a diameter of 1.3-2.0 micrometers were observed singularly or in pairs, short chains and irregular conglomerates. Growth occurred within the pH range 6.0-10.0, with optimum growth at pH 8.5. The temperature range for growth of the novel isolate was from -5 to 35 C, with optimum growth at 28-30 C. Strain PmagG1(sup T) did not require NaCl, as growth was observed in the presence of 0-6.5% NaCl with optimum growth at 0.5% (w/v). Strain PmagGl(sup T) was a catalase-negative chemo-organoheterotroph that used sugars and some organic acids as substrates. The metabolic end products were lactate, formate, acetate, ethanol and Con. Strain PmagG1(sup T) was sensitive to ampicillin, tetracycline, chloramphenicol, rifampicin, kanamycin and gentamicin. The G+C content of its genomic DNA was 45.8 mol%. 16S rRNA gene sequence analysis showed 100 % similarity of strain PmagG1(sup T) with Trichococcus collinsii ATCC BAA-296(sup T), but DNA-DNA hybridization between them demonstrated relatedness values of less than 45 plus or minus 1%. Another phylogenetically closely related species, Trichococcus pasteurii, showed 99.85 % similarity by 16s rRNA sequencing and DNA-DNA hybridization showed relatedness values of 47 plus or minus 1.5%. Based on genotypic and phenotypic characteristics, the novel species Trichococcus patagoniensis sp. nov. is proposed, with strain PmagG1(sup T) (=ATCC BAA-756(sup T)=JCM 12176(sup T)=CIP 108035(sup T)) as the type strain.
Document ID
20070004782
Acquisition Source
Marshall Space Flight Center
Document Type
Reprint (Version printed in journal)
Authors
Pikuta, Elena V.
(NASA Marshall Space Flight Center Huntsville, AL, United States)
Hoover, Richard B.
(NASA Marshall Space Flight Center Huntsville, AL, United States)
Bej, Asim K.
(Alabama Univ. Birmingham, AL, United States)
Marsic, Damien
(Alabama Univ. Huntsville, AL, United States)
Whitman, William B.
(Georgia Univ. Athens, GA, United States)
Krader, Paul E.
(American Type Culture Collection Manassas, VA, United States)
Tang, Jane
(Mitretek Systems, Inc. Falls Church, VA, United States)
Date Acquired
August 24, 2013
Publication Date
January 1, 2006
Publication Information
Publication: International Journal of Systematic and Evolutionary Microbiology
Volume: 56
Subject Category
Life Sciences (General)
Distribution Limits
Public
Copyright
Other

Available Downloads

There are no available downloads for this record.
No Preview Available