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Record 1 of 32501
Break Point Distribution on Chromosome 3 of Human Epithelial Cells exposed to Gamma Rays, Neutrons and Fe Ions
Author and Affiliation:
Hada, M.(NASA Johnson Space Center, Houston, TX, United States)
Saganti, P. B.(Prairie View Agricultural and Mechanical Coll., TX, United States)
Gersey, B.(Prairie View Agricultural and Mechanical Coll., TX, United States)
Wilkins, R.(Prairie View Agricultural and Mechanical Coll., TX, United States)
Cucinotta, F. A.(NASA Johnson Space Center, Houston, TX, United States)
Wu, H.(NASA Johnson Space Center, Houston, TX, United States)
Abstract: Most of the reported studies of break point distribution on the damaged chromosomes from radiation exposure were carried out with the G-banding technique or determined based on the relative length of the broken chromosomal fragments. However, these techniques lack the accuracy in comparison with the later developed multicolor banding in situ hybridization (mBAND) technique that is generally used for analysis of intrachromosomal aberrations such as inversions. Using mBAND, we studied chromosome aberrations in human epithelial cells exposed in vitro to both low or high dose rate gamma rays in Houston, low dose rate secondary neutrons at Los Alamos National Laboratory and high dose rate 600 MeV/u Fe ions at NASA Space Radiation Laboratory. Detailed analysis of the inversion type revealed that all of the three radiation types induced a low incidence of simple inversions. Half of the inversions observed after neutron or Fe ion exposure, and the majority of inversions in gamma-irradiated samples were accompanied by other types of intrachromosomal aberrations. In addition, neutrons and Fe ions induced a significant fraction of inversions that involved complex rearrangements of both inter- and intrachromosome exchanges. We further compared the distribution of break point on chromosome 3 for the three radiation types. The break points were found to be randomly distributed on chromosome 3 after neutrons or Fe ions exposure, whereas non-random distribution with clustering break points was observed for gamma-rays. The break point distribution may serve as a potential fingerprint of high-LET radiation exposure.
Publication Date: Jan 01, 2007
Document ID:
20070021585
(Acquired Jul 11, 2007)
Subject Category: LIFE SCIENCES (GENERAL)
Document Type: Conference Paper
Financial Sponsor: NASA Johnson Space Center; Houston, TX, United States
Organization Source: NASA Johnson Space Center; Houston, TX, United States
Description: 1p; In English
Distribution Limits: Unclassified; Publicly available; Unlimited
Rights: Copyright
NASA Terms: CHROMOSOME ABERRATIONS; GAMMA RAYS; EXTRATERRESTRIAL RADIATION; EXPOSURE; RADIATION DOSAGE; IRRADIATION; CHROMOSOMES; IN VITRO METHODS AND TESTS; STATISTICAL DISTRIBUTIONS
Miscellaneous Notes: 18th annual meeting, July 13-15, 2007, Rohnert Park, CA
Availability Source: Other Sources
Availability Notes: Abstract Only
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