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The Effects of Modeled Microgravity on Nucleocytoplasmic Localization of Human Apurinic/ApyrimidinicExposure to space radiation and microgravity occurs to humans during space flight. In order to have accurate risk estimations, answering questions to whether increased DNA damage seen during space flight in modified by microgravity are important. Several studies have examined whether intercellular repair of radiation-induced DNA lesions are modified by microgravity. Results from these studies show no modification of the repair processes due to microgravity. However, it is known that in studies not involving radiation that microgravity interferes with normal development. Interestingly, there is no data that attempts to analyze the possible effects of microgravity on the trafficking of DNA repair proteins. In this study, we analyze the effects of modeled microgravity on nucleocytoplasmic shuttling of the human DNA repair enzyme apurinic/apyrimidinic endonuclease 1 (APE1/Ref1) which is involved in base excision repair. We examined nuclear translocation of APE1 using enhanced green fluorescent protein (EGFP) fused to APE1 as a reporter. While APE1 under normal gravity showed normal nuclear localization, APE1 nuclear localization under modeled microgravity was decreased. These results suggest that nucleocytoplasmic translocation of APE1 is modified under modeled microgravity.
Document ID
20080026161
Acquisition Source
Johnson Space Center
Document Type
Conference Paper
Authors
Gonda, Steve
(NASA Johnson Space Center Houston, TX, United States)
Jackson, E.B.
(Texas Univ. Galveston, TX, United States)
Date Acquired
August 24, 2013
Publication Date
February 23, 2004
Subject Category
Aerospace Medicine
Meeting Information
Meeting: 2005 NASA Cell Science Conference
Location: Moody Gardens, Galveston, Texas
Country: United States
Start Date: February 23, 2004
End Date: February 25, 2004
Sponsors: NASA Headquarters
Funding Number(s)
OTHER: NASA101-31-GN-DA
Distribution Limits
Public
Copyright
Work of the US Gov. Public Use Permitted.

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