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Inter- and Intra-Chromosomal Aberrations in Human Cells Exposed in vitro to High and Low LET RadiationsEnergetic heavy ions pose a health risk to astronauts in extended ISS and future Mars missions. High-LET heavy ions are particularly effective in causing various biological effects including cell inactivation, genetic mutations and cancer induction. Most of these biological endpoints are closely related to chromosomal damage, which can be utilized as a biomarker for radiation insults. Previously, we had studied chromosome aberrations in human lymphocytes and fibroblasts induced by both low- and high-LET radiation using FISH and multicolor fluorescence in situ hybridization (mFISH) techniques. In this study, we exposed human epithelial cells in vitro to gamma rays and energetic particles of varying types and energies and dose rates, and analyzed chromosomal damages using the multicolor banding in situ hybridization (mBAND) procedure. Confluent human epithelial cells (CH184B5F5/M10) were exposed to energetic heavy ions at NASA Space Radiation Laboratory (NSRL) at the Brookhaven National Laboratory, high energy neutron at the Los Alamos Nuclear Science Center (LANSCE) or Cs-137-gamma radiation source at the University of Texas, MD Anderson Cancer Center. After colcemid and Calyculin A treatment, cells were fixed and painted with XCyte3 mBAND kit (MetaSystems) and chromosome aberrations were analyzed with mBAND analysis system (MetaSystems). With this technique, individually painted chromosomal bands on one chromosome allowed the identification of interchromosomal aberrations (translocation to unpainted chromosomes) and intrachromosomal aberrations (inversions and deletions within a single painted chromosome). The results of the mBAND study showed a higher ratio of inversion involved with interchromosomal exchange in heavy ions compared to -ray irradiation. Analysis of chromosome aberrations using mBAND has the potential to provide useful information on human cell response to space-like radiation.
Document ID
20080029372
Acquisition Source
Johnson Space Center
Document Type
Conference Paper
Authors
Hada, M.
(NASA Johnson Space Center Houston, TX, United States)
Wilkins, R.
(Prairie View Agricultural and Mechanical Coll. TX, United States)
Saganti, P. B.
(Prairie View Agricultural and Mechanical Coll. TX, United States)
Gersey, B.
(Prairie View Agricultural and Mechanical Coll. TX, United States)
Cucinotta, F. A.
(NASA Johnson Space Center Houston, TX, United States)
Wu, H.
(NASA Johnson Space Center Houston, TX, United States)
Date Acquired
August 24, 2013
Publication Date
July 23, 2006
Subject Category
Life Sciences (General)
Meeting Information
Meeting: Committee on Space Research (COSPAR) Colloquium
Location: Xian
Country: China
Start Date: July 23, 2006
End Date: July 25, 2006
Sponsors: Harbin Inst. of Tech.
Distribution Limits
Public
Copyright
Work of the US Gov. Public Use Permitted.

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