Novel Confocal Microscopic and Flow Cytometric Based Assays to Visualize and Detect the (Beta)2-Adrenergic Receptor in Human Lymphocyte and Mononuclear Cell PopulationsThe data show that immunophenotyping of leukocyte populations with (beta)2AR is possible with the commercially available Ab, although the FC assay is limited to the IST as a result of the Ab binding site to the intracellular C-terminus of the 2AR. The FC assay has applications for measuring alterations in total (beta)2AR in human leukocyte populations as changes in fluorescence. In addition, CM confirms that both surface and intracellular compartments stain positively for the (beta)2AR and can be used for qualitative assays that screen for changes in receptor compartmentalization and localization.
Document ID
20080030263
Acquisition Source
Johnson Space Center
Document Type
Conference Paper
Authors
Salicru, A. N. (Texas Univ. Health Science Center Houston, TX, United States)
Crucian, B. E. (Wyle Labs., Inc. Houston, TX, United States)
Nelman, M. A. (Wyle Labs., Inc. Houston, TX, United States)
Sams, C. F. (NASA Johnson Space Center Houston, TX, United States)
Actor, J. K. (NASA Johnson Space Center Houston, TX, United States)
Marshall, G. D. (Mississippi Univ. Medical Center Jackson, MS, United States)