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Record Details

Record 14 of 3389
Optimization of PMA-PCR Protocol for Viability Detection of Pathogens
External Online Source: hdl:2014/42244
Author and Affiliation:
Mikkelson, Brian J.(North Dakota State Univ., Fargo, ND, United States)
Lee, Christine M.(Jet Propulsion Lab., California Inst. of Tech., Pasadena, CA, United States)
Ponce, Adrian(Jet Propulsion Lab., California Inst. of Tech., Pasadena, CA, United States)
Abstract: This presented study demonstrates the need that PMA-PCR can be used to capture the loss of viability of a sample that is much more specific and time-efficient than alternative methods. This protocol is particularly useful in scenarios in which sterilization treatments may inactivate organisms but not degrade their DNA. The use of a PCR-based method of pathogen detection without first inactivating the DNA of nonviable cells will potentially lead to false positives. The loss of culturability, by heat-killing, did not prevent amplified PCR products, which supports the use of PMA to prevent amplification and differentiate between viable and dead cells. PMA was shown to inhibit the amplification of DNA by PCR in vegetative cells that had been heat-killed.
Publication Date: Aug 01, 2011
Document ID:
20120015246
(Acquired Oct 26, 2012)
Subject Category: LIFE SCIENCES (GENERAL)
Document Type: Technical Report
Financial Sponsor: Jet Propulsion Lab., California Inst. of Tech.; Pasadena, CA, United States
Organization Source: Jet Propulsion Lab., California Inst. of Tech.; Pasadena, CA, United States
Description: 8p; In English; Original contains black and white illustrations
Distribution Limits: Unclassified; Publicly available; Unlimited
Rights: Copyright
NASA Terms: DEOXYRIBONUCLEIC ACID; STERILIZATION; PATHOGENS; ORGANISMS; DEACTIVATION; VIABILITY; PROTOCOL (COMPUTERS); DETECTION; AMPLIFICATION
Other Descriptors: PROPIDIUM MONOAZIDE (PMA); MOLECULAR BIOLOGY
Availability Source: Other Sources
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Last Modified: October 26, 2012
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