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Spaceflight Effects and Molecular Responses in the Mouse Eye: Observations After Shuttle Mission STS-133Microgravity-induced cephalad fluid shift and radiation exposure are some of the stressors seen in space exploration. Ocular changes leading to visual impairment in astronauts are of occupational health relevance. Therefore, we analyzed the effects of space flight in the eyes of mice. Six mice were assigned to Flight (FLT), Animal enclosure Module (AEM), or vivarium (VIV) group, respectively. Mice were sacrificed at 1, 5 or 7 days after landing from space. One eye was used for histological and immunohistoche-mistry analysis and the other eye for gene expression profiling. 8-OHdG and caspase-3 immunoreactivity were increased in the retina in FLT samples at return(R+1) compared to AEM/VIV groups, and decreased at day 7 (R+7). beta-amyloid was seen in the nerve fibers at the post-laminar region of the optic nerve in the flight samples (R+7). In addition, oxidative and cellular stress response genes were upregulated in the retina of FLT samples upon landing, and decreased by R+7. According to the results, a reversible molecular damage may occur in the retina of mice exposed to spaceflight followed by protective cellular response.
Document ID
Acquisition Source
Johnson Space Center
Document Type
Prospero-Ponce, Claudia
(Methodist Hospital Houston, TX, United States)
Zanello, Susana B.
(Universities Space Research Association Houston, TX, United States)
CoreyTheriot, Patricia
(Wyle Integrated Science and Engineering Group Houston, TX, United States)
Chevez-Barrios, P.
(Methodist Hospital Houston, TX, United States)
Date Acquired
August 25, 2013
Publication Date
November 12, 2012
Subject Category
Aerospace Medicine
Report/Patent Number
Meeting Information
Meeting: American Association of Ophthalmic (AAOOP)
Location: Chicago, IL
Country: United States
Start Date: November 9, 2012
Distribution Limits
Public Use Permitted.
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