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Dependence of Early and Late Chromosomal Aberrations on Radiation Quality and Cell TypesExposure to radiation induces different types of DNA damage, increases mutation and chromosome aberration rates, and increases cellular transformation in vitro and in vivo. The susceptibility of cells to radiation depends on genetic background and growth condition of cells, as well as types of radiation. Mammalian cells of different tissue types and with different genetic background are known to have different survival rate and different mutation rate after cytogenetic insults. Genomic instability, induced by various genetic, metabolic, and environmental factors including radiation, is the driving force of tumorigenesis. Accurate measurements of the relative biological effectiveness (RBE) is important for estimating radiation-related risks. To further understand genomic instability induced by charged particles and their RBE, we exposed human lymphocytes ex vivo, human fibroblast AG1522, human mammary epithelial cells (CH184B5F5/M10), and bone marrow cells isolated from CBA/CaH(CBA) and C57BL/6 (C57) mice to high energy protons and Fe ions. Normal human fibroblasts AG1522 have apparently normal DNA damage response and repair mechanisms, while mammary epithelial cells (M10) are deficient in the repair of DNA DSBs. Mouse strain CBA is radio-sensitive while C57 is radio-resistant. Metaphase chromosomes at different cell divisions after radiation exposure were collected and chromosome aberrations were analyzed as RBE for different cell lines exposed to different radiations at various time points up to one month post irradiation.
Document ID
20170001349
Acquisition Source
Johnson Space Center
Document Type
Presentation
Authors
Lu, Tao
(Universities Space Research Association Houston, TX, United States)
Zhang, Ye
(NASA Kennedy Space Center Cocoa Beach, FL, United States)
Krieger, Stephanie
(KBRwyle Science, Technology and Engineering Houston, TX, United States)
Yeshitla, Samrawit
(NASA Johnson Space Center Houston, TX, United States)
Goss, Rosalin
(Texas Southern Univ. Houston, TX, United States)
Bowler, Deborah
(Oxford Brookes Univ. Headington, United Kingdom)
Kadhim, Munira
(Oxford Brookes Univ. Headington, United Kingdom)
Wilson, Bobby
(Texas Southern Univ. Houston, TX, United States)
Rohde, Larry
(Houston Univ.-Clear Lake Houston, TX, United States)
Wu, Honglu
(NASA Johnson Space Center Houston, TX, United States)
Date Acquired
February 7, 2017
Publication Date
January 23, 2017
Subject Category
Aerospace Medicine
Life Sciences (General)
Report/Patent Number
JSC-CN-38623
Meeting Information
Meeting: 2017 NASA Human Research Program Investigators'' Workshop (HRP IWS 2017) Annual Meeting
Location: Glaveston, TX
Country: United States
Start Date: January 23, 2017
End Date: January 26, 2017
Sponsors: NASA Johnson Space Center, National Space Biomedical Research Inst. (NSBRI)
Distribution Limits
Public
Copyright
Public Use Permitted.
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