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Abiotic Synthesis of Nucleic Acids: Hypochromicity and Future Research
NTRS Full-Text: Click to View  [PDF Size: 1.6 MB]
Author and Affiliation:
Glass, K.(California Univ., San Diego, CA, United States)
Oye, M.(California Univ., Santa Cruz, CA, United States)
Deamer, D.(California Univ., Santa Cruz, CA, United States)
Vercoutere, W.(NASA Ames Research Center, Moffett Field, CA, United States)
Abstract: The earliest forms of life would likely have a protocellular form, with a membrane encapsulating some form of linear charged polymer. These polymers could have enzymatic as well as genetic properties. We can simulate plausible prebiotic conditions in the laboratory to test hypotheses related to this concept. In earlier work we have shown that mononucleotides organized within a multilamellar lipid matrix can produce oligomers in the anhydrous phase of dehydration- rehydration cycles (Rajamani, 2008). If mononucleotides are in solution at millimolar concentrations, then oligomers resembling RNA are synthesized and exist in a steady state with their monomers DeGuzman, 2014). We have used conventional and novel techniques to demonstrate that secondary structures stabilized by hydrogen bonds may be present in the condensation products produced in dehydration- rehydration cycles that simulate hydrothermal fields that were present on the early Earth. Gel electrophoresis data corroborates the presence of up to 200-base pair length RNA fragments in products of Hydration-Dehydration experiments. Furthermore, hypochromicity measurements demonstrate a degree of hypochromicity found in single RNA strand of known sequence, as well as results that indicate this is true also for a sample of complementary strands of RNA. Analysis of ionic current signatures of known RNA hairpin molecule as measured using a nanopore detector indicate a significant variability in pattern, different from the signatures produced by DNA hairpin molecules. This informs how we may interpret nanopore data gathered from prebiotic simulations.
Publication Date: Jul 16, 2017
Document ID:
(Acquired Dec 13, 2017)
Report/Patent Number: ARC-E-DAA-TN44794
Document Type: Oral/Visual Presentation
Meeting Information: 18th International Conference on the Origin of Life; 16-21 Jul. 2017; San Diego, CA; United States
Meeting Sponsor: Universities Space Research Association; Moffett Field, CA, United States
Earth Life Science Institute; Tokyo, Japan
NASA Ames Research Center; Moffett Field, CA, United States
Financial Sponsor: NASA Ames Research Center; Moffett Field, CA, United States
Organization Source: NASA Ames Research Center; Moffett Field, CA, United States
Description: 1p; In English
Distribution Limits: Unclassified; Publicly available; Unlimited
Rights: Copyright; Public use permitted
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