Developing a Tool to Collect Microbiological Samples in Space

We developed and tested a tool to collect aseptic samples during extravehicular activity or space walks. This tool can withstand the extreme temperature (-92 – 90 ˚C) and pressure variations without compromising the sterility of the collection device or the integrity of the sample. Results from ground testing the tool demonstrate that external surfaces of space suits are not sterile and that a wide variety of human associated bacteria and some fungi can survive at least seven hours of exposure to vacuum. These bacteria include non-spore forming organisms like Staphylococcus that are not normally considered extremophiles We plan to use this tool to characterize the exterior of the International Space Station. We expect the results of this work to guide the planetary protection requirements and contamination control strategy for crewed missions to Mars. This tool could also be used to monitor contamination associated with the Artemis missions to the moon or collect aseptic samples robotically in environments that are not suitable for human exploration.