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Continuous flow electrophoresis system experiments on shuttle flights STS-6 and STS-7A space continuous flow electrophoresis system (CFES) was developed that would incorporate specific modifications to laboratory instruments to take advantage of weightlessness. The specific objectives were to use a model sample material at a high concentration to evaluate the continuous flow electrophoresis process in the CFES instrument and compare its separation resolution and sample throughput with related devices on Earth and to expand the basic knowledge of the limitations imposed by fluid flows and particle concentration effects on the electrophoresis process by careful design and evaluation of the space experiment. Hemoglobin and polysaccharide were selected as primary samples. The results from space show a large band spread of the high concentration of the single species of hemoglobin that was due to the mismatch of electrical conductivity between the sample and the buffer. On STS-7 the major objective was to evaluate the influence of the electrical properties of the sample constituents on the resolution of the CFES. As expected, the polystyrene latex microspheres dispersed in a solution with 3 times the electrical conductivity of the curtain buffer separated with a larger band spread than in the 2nd experiment.
Document ID
19880001596
Document Type
Technical Publication (TP)
Authors
Snyder, Robert S. (NASA Marshall Space Flight Center Huntsville, AL, United States)
Rhodes, Percy H. (NASA Marshall Space Flight Center Huntsville, AL, United States)
Miller, Teresa Y. (NASA Marshall Space Flight Center Huntsville, AL, United States)
Date Acquired
September 5, 2013
Publication Date
October 1, 1987
Subject Category
MATERIALS PROCESSING
Report/Patent Number
NASA-TP-2778
NAS 1.60:2778
Distribution Limits
Public
Copyright
Work of the US Gov. Public Use Permitted.

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